Faculty


Ashish K. Singh

Assistant Professor

Mucosal and Innate Immunology
Email: ashish.singh.acbr@gmail.com
ORCID: https://orcid.org/0000-0002-4633-1513

Dr. Ashish Kumar Singh is an Assistant Professor at the Dr. B. R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi, North Campus. He received his PhD in 2015 from The Maharaja Sayajirao University of Baroda, Vadodara. As part of his doctoral studies, he was awarded the prestigious European Commission Erasmus Mundus mobility fellowship in 2014, enabling him to spend one year as a visiting PhD researcher at University College Dublin (UCD), Ireland.Following the completion of his PhD, Dr. Singh started postdoctoral training at the UCD Conway Institute, Ireland, with Professor Ulla Knaus. During his postdoctoral research tenure spanning more than nine years at the institute, he led and contributed to several projects in the domain of Mucosal immunology, Host-microbe interactions, NADPH oxidase biology, Inflammatory bowel disease (IBD), and Neutrophil plasticity & functional reprogramming.

Dr. Singh is currently establishing the Laboratory of Mucosal and Innate Immunology at ACBR, with a contemporary research program dedicated to unravelling the cellular and molecular mechanisms that govern neutrophil reprogramming during infection and inflammation.By mapping the molecular and metabolic pathways that drive neutrophil transitions during infection and inflammation, the laboratory aims to identify key regulatory factors that determine whether neutrophils adopt protective or pathological roles. These insights are expected to inform the development of precision immunotherapies that modulate innate immune responses without compromising host defense.In parallel, the laboratory also aims to investigate innate immune defense mechanisms of mucosal tissues, with a particular focus on the intestinal epithelium. The goal is to identify critical molecular and physiological determinants underlying susceptibility to inflammatory bowel disease (IBD) and enteric infections, contributing to a deeper understanding of mucosal immunity in health and disease.

Selected Recent Publications:
  1. 1.Singh, A.K.*, Blanco, A., Sinnott, R. and Knaus, U.G. (2025). Rapid Isolation and Flow Cytometry Analysis of Murine Intestinal Immune Cells After Chemically Induced Colitis. Bio-protocol 15(16): e5417.*Corresponding author.10.21769/BioProtoc.5417.

  2. 2.Singh, A.K., Ainciburu, M., Wynne, K., Bhat, S.A., Blanco, A., Tzani, I., Akiba, Y., Lalor, S.J., Kaunitz, J., Bourke, B. and Kelly, V.P., Doherty, G.A., Zerbe, C.S., Clarke, C., Hussey, S., and Knaus, U.G., 2025. De novo DUOX2 expression in neutrophil subsets shapes the pathogenesis of intestinal disease. Proc. Natl. Acad. Sci. U.S.A. 122 (19), e2421747122. https://doi.org/10.1073/pnas.2421747122

  3. 3.Saldova, R., Thomsson, K.A., Wilkinson, H., Chatterjee, M., Singh, A.K., Karlsson, N.G. and Knaus, U.G., 2024. Characterization of intestinal O-glycome in reactive oxygen species deficiency. Plos one, 19(3), p.e0297292. https://doi.org/10.1371/journal.pone.0297292

  4. 4.Stenke, E., Aviello, G., Singh, A.K., Martin, S., Winter, D., Sweeney, B., McDermott, M., Bourke, B., Hussey, S., and Knaus, U.G., 2020. NADPH oxidase 4 is protective and not fibrogenic in intestinal inflammation. Redox Biology, 37, p.101752. https://doi.org/10.1016/j.redox.2020.101752

  5. 5.Jennings, R.T.*, Singh, A.K.* and Knaus, U.G., 2019. Redox regulator network in inflammatory signaling. Current Opinion in Physiology, 9, pp.9-17. https://doi.org/10.1016/j.cophys.2019.03.002. (*equal contributions)https://doi.org/10.1016/j.redox.2020.101752

  6. 6.Aviello, G., Singh, A.K., O'Neill, S., Conroy, E., Gallagher, W., D'Agostino, G., Walker, A.W., Bourke, B., Scholz, D. and Knaus, U.G., 2019. Colitis susceptibility in mice with reactive oxygen species deficiency is mediated by mucus barrier and immune defense defects. Mucosal immunology, 12(6), pp.1316-1326. https://doi.org/10.1038/s41385-019-0205-x

  7. 7.Singh, A.K., Hertzberger, R.Y. and Knaus, U.G., 2018. Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis. Redox Biology, 16, pp.11-20. https://doi.org/10.1016/j.redox.2018.02.003

  8. 8.Singh, A.K., Pandey, S.K., Saha, G. and Gattupalli, N.K., 2015. Pyrroloquinoline quinone (PQQ) producing Escherichia coli Nissle 1917 (EcN) alleviates age associated oxidative stress and hyperlipidemia, and improves mitochondrial function in ageing rats. Experimental Gerontology, 66, pp.1-9. https://doi.org/10.1016/j.exger.2015.04.001

  9. 9.Pandey, S., Singh, A. K., Chaudhari, N., Nampoothiri, L.P. and Kumar, G.N., 2015. Protection against 1, 2-di-methylhydrazine-induced systemic oxidative stress and altered brain neurotransmitter status by probiotic Escherichia coli CFR 16 secreting pyrroloquinoline quinone. Current Microbiology, 70, pp.690-697. https://doi.org/10.1007/s00284-014-0763-9

  10. 10.Singh, A.K., Pandey, S.K. and Naresh Kumar, G., 2014. Pyrroloquinoline Quinone‐Secreting Probiotic Escherichia coli Nissle 1917 Ameliorates Ethanol‐Induced Oxidative Damage and Hyperlipidemia in Rats. Alcoholism: Clinical and Experimental Research, 38(7), pp.2127-2137. https://doi.org/10.1111/acer.12456

  11. 11.Pandey, S., Singh, A. K., Kumar, P., Chaudhari, A. and Nareshkumar, G., 2014. Probiotic Escherichia coli CFR 16 producing pyrroloquinoline quinone (PQQ) ameliorates 1, 2-dimethylhydrazine-induced oxidative damage in colon and liver of rats. Applied Biochemistry and Biotechnology, 173, pp.775-786. https://doi.org/10.1007/s12010-014-0897-z