Faculty


Ashish K. Singh

Assistant Professor

Mucosal and Innate Immunology
Email: ashish.singh.acbr@gmail.com
ORCID: https://orcid.org/0000-0002-4633-1513

Dr. Ashish Kumar Singh is an Assistant Professor at the Dr. B. R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi, where he leads the Laboratory of Mucosal and Innate Immunology. He earned his Ph.D. in 2015 from The Maharaja Sayajirao University of Baroda, which included an Erasmus Mundus fellowship as a visiting researcher at University College Dublin (UCD). Following his doctorate, he completed over nine years of postdoctoral research at the School of Medicine, UCD, specializing in mucosal immunology, host-microbe interactions, and neutrophil biology.

His current research explores the molecular mechanisms driving neutrophil reprogramming during infection and inflammation, as well as mucosal defense mechanisms in inflammatory bowel disease (IBD) and enteric infections, aiming to advance precision immunotherapies.

Honors and Awards:

  1. Identifying intracellular pathways that govern neutrophil reprogramming to a proinflammatory DUOX2+ phenotype in inflammatory bowel disease. Anusandhan National Research Foundation (ANRF), GoI, 2026-2029. Sanctioned Amount: 67,35,120 INR.

Selected Recent Publications:
  1. 1.Rius-Pérez, S., Jiménez-Cañete, N., Singh, A.K., Gil, A., Benitez-Martínez, J., Noguera, I., Blanch, E., Therry, N., Pinto, S., Knaus, U.G. and Sastre, J., 2026. Divergent roles of NOX1 and NOX2 in acute pancreatitis and downregulation of NOX2 in obesity. Redox Biology, p.104313. https://doi.org/10.1016/j.redox.2026.104313.

  2. 2.Singh, A. K., Mara, M., Drieu La Rochelle, J., Therry, N., D'Alessio, A., Baugh, J., Barre, R. S., Matsumoto, M., Nogales, A., Martínez-Sobrido, L., & Knaus, U. G. (2025). Emergence of NOX1/DUOX NADPH oxidases is a key feature of functional neutrophil reprogramming at the gut and lung barrier. Redox Biology, 87, 103883. https://doi.org/10.1016/j.redox.2025.103883.

  3. 3.Singh, A. K., Blanco, A., Sinnott, R., & Knaus, U. G. (2025). Rapid Isolation and Flow Cytometry Analysis of Murine Intestinal Immune Cells After Chemically Induced Colitis. Bio-protocol, 15(16), e5417. https://doi.org/10.21769/BioProtoc.5417.

  4. 4.Singh, A. K., Ainciburu, M., Wynne, K., Bhat, S. A., Blanco, A., Tzani, I., Akiba, Y., Lalor, S. J., Kaunitz, J., Bourke, B., Kelly, V. P., Doherty, G. A., Zerbe, C. S., Clarke, C., Hussey, S., & Knaus, U. G. (2025). De novo DUOX2 expression in neutrophil subsets shapes the pathogenesis of intestinal disease. Proceedings of the National Academy of Sciences of the United States of America, 122(19), e2421747122. https://doi.org/10.1073/pnas.2421747122.

  5. 5.Saldova, R., Thomsson, K. A., Wilkinson, H., Chatterjee, M., Singh, A. K., Karlsson, N. G., & Knaus, U. G. (2024). Characterization of intestinal O- glycome in reactive oxygen species deficiency. PloS one, 19(3), e0297292. https://doi.org/10.1371/journal.pone.0297292.

  6. 6.Stenke, E., Aviello, G., Singh, A., Martin, S., Winter, D., Sweeney, B., McDermott, M., Bourke, B., Hussey, S., & Knaus, U. G. (2020). NADPH oxidase 4 is protective and not fibrogenic in intestinal inflammation. Redox Biology, 37, 101752. https://doi.org/10.1016/j.redox.2020.101752.

  7. 7.Aviello, G., Singh, A. K., ;Neill, S., Conroy, E., Gallagher, W., D'Agostino, G., Walker, A. W., Bourke, B., Scholz, D., & Knaus, U. G. (2019). Colitis susceptibility in mice with reactive oxygen species deficiency is mediated by mucus barrier and immune defense defects. Mucosal immunology, 12(6), 1316-1326. https://doi.org/10.1038/s41385-019-0205-x.

  8. 8.Jennings, R. T.*, Singh, A. K.*, & Knaus, U. G. (2019). Redox regulator network in inflammatory signaling. Current Opinion in Physiology, 9, 9-17.

  9. 9.Singh, A. K., Hertzberger, R. Y., & Knaus, U. G. (2018). Hydrogen peroxide production by lactobacilli promotes epithelial restitution during colitis. Redox Biology, 16, 11-20. https://doi.org/10.1016/j.redox.2018.02.003.

  10. 10.Singh, A. K., Pandey, S. K., Saha, G., & Gattupalli, N. K. (2015). Pyrroloquinoline quinone (PQQ) producing Escherichia coli Nissle 1917 (EcN) alleviates age associated oxidative stress and hyperlipidemia, and improves mitochondrial function in ageing rats. Experimental gerontology, 66, 1-9. https://doi.org/10.1016/j.exger.2015.04.001.

  11. 11.Pandey, S., Singh, A., Chaudhari, N., Nampoothiri, L. P., & Kumar, G. N. (2015). Protection against 1,2-di-methylhydrazine-induced systemic oxidative stress and altered brain neurotransmitter status by probiotic Escherichia coli CFR 16 secreting pyrroloquinoline quinone. Current Microbiology, 70(5), 690-697. https://doi.org/10.1007/s00284-014-0763-9.

  12. 12.Singh, A. K., Pandey, S. K., & Naresh Kumar, G. (2014). Pyrroloquinoline quinone-secreting probiotic Escherichia coli Nissle 1917 ameliorates ethanol-induced oxidative damage and hyperlipidemia in rats. Alcoholism, clinical and experimental research, 38(7), 2127-2137. https://doi.org/10.1111/acer.12456.

  13. 13.Pandey, S., Singh, A., Kumar, P., Chaudhari, A., & Nareshkumar, G. (2014). Probiotic Escherichia coli CFR 16 producing pyrroloquinoline quinone (PQQ) ameliorates 1,2-dimethylhydrazine-induced oxidative damage in colon and liver of rats. Applied biochemistry and biotechnology, 173(3), 775-786. https://doi.org/10.1007/s12010-014-0897-z.