Laishram R. Singh

Professor

Protein Biochemistry & Molecular Biophysics
Email: lairksingh@gmail.com

Neurodegenerative disorders are becoming a significant cause for the increasing proportion of mortality in the developed world. One of the common hallmark and unifying characteristic of all neurodegenerative diseases is the presence of toxic protein deposits (oligomers) either intracellularly or extracellularly. In recent years an increased level of homocysteine in the blood plasma has been linked with the promotion of neurodegenerative disorders. The exact mechanism how protein deposits or increase in the level of homocysteine leads to neuronal death (neurodegenerative diseases) has not been unveiled. Currently, Dr. Singh’s laboratory is interested in investigating neurodegenerative disorders caused due to homocysteine toxicity and due to toxic protein deposits in neuronal cells. In another project the laboratory is also interested in using proteostatic modulators (drugs that can modulate protein quality control system) for the therapeautic intervention of leukemia.

Ongoing Projects:

1. 1. “Effect of multiple osmolytes on protein stability, structure and function : An insight to additivity, synergism or antagonism of osmolytes mixtures. (CSIR, 2014-2017)
   
   
2. 2. “Protein covalent modification by homocyteine: Understanding the reactivity, toxicity, structural and functional consequences. (DBT,2016-2019)
   
   

Selected Recent Publications:

1. 1. Kumari, Kritika; Warepam, Marina; Aniket, Bansal; Dar, TA; VN, Uversky and Singh L. R. The gut metabolite, trimethylamine N-oxide inhibits protein folding by affecting cis-trans isomerization and induces cell cycle arrest. Cellular and Molecular Life Sciences (2022) 79, 1 12. (Impact factor - 9.261)
   
   
2. 2. Sharma, Gurumayum Suraj; Bhattacharya, Reshmee; Krishna, Snigdha; Alomar, Suliman Y.; Alkhuriji, Afrah; Warepam, Marina; Kumari, Kritika; Rahaman, Hamidur; Singh, Laishram. Structural and Functional Characterization of Covalently Modified Proteins Formed By Glycating Agent, Glyoxal. ACS Omega (2021) 6, 32, 20887-20894 (Impact factor - 3.51 ISSN 2470-1343)
   
   
3. 3. Gurumayum Suraj Sharma, Reshmee Bhattacharya, Laishram Rajendrakumar Singh. Functional inhibition of redox regulated heme proteins: A novel mechanism towards oxidative stress induced by homocysteine. Redox Biology (2021) 46, 102080 (Impact factor - 11.79 ISSN 2213-2317)
   
   
4. 4. Angamba Meetei Potshangbam, Apana Nandeibam, Thongamba Amom, Nongdam Potshangbam, Hamidur Rahaman, Ravindranath Singh Rathore, Laishram Rajendrakumar Singh, Aslam Khan. An in silico approach to identify potential medicinal plants for treating Alzheimer disease: a case study with acetylcholinesterase. J. Biomol. Struct. Des. (2020) 1521-1533
   
   
5. 5. Chowhan R.K., Rahaman H., Singh L.R. Structural basis of Peroxidase Catalytic Cycle of human Prdx6. Sci Rep. (2020) 10:17416
   
   
6. 6. Mohd Younus Bhat, Laishram Rajendrakumar Singh, Tanveer Ali Dar. Taurine Induces an Ordered but Functionally Inactive Conformation in Intrinsically Disordered Casein Proteins. Sci Rep. (2020) 10: 3503.
   
   
7. 7. Mudasir Ahmad Rather, Tanveer Ali Dar, Laishram R. Singh, Ghulam Mohammad Rather Mohsin Ahmad Bhat, Structural-functional integrity of lysozyme in imidazolium based surface active ionic liquids. Int. J. Biol. Macromol. 2020, 15: 271-279
   
   
8. 8. Arif Bashir, Younis Hazari, Debnath Pal, Dibyajyoti Maity, Samirul Bashir, Laishram Rajendrakumar Singh, Naveed Nazir Shah, Khalid Majid Fazili. Aggregation of M3 (E376D) variant of alpha1- antitrypsin. Sci Rep. 2020; 10: 8290.
   
   
9. 9. Marina Warepam, Khurshid Ahmad, Safikur Rahman, Hamidur Rahaman, Kritika Kumari, Laishram Rajendrakumar Singh. N-Acetylaspartate Is an Important Brain Osmolyte. Biomolecules. 2020 Feb; 10(2): 286.
   
   
10. 10.Shahnaj S, Chowhan RK, Meetei PA, Kakchingtabam P, Herojit Singh K, Rajendrakumar Singh L, Nongdam P, Fisher AB, Rahaman H. Hyperoxidation of Peroxiredoxin 6 Induces Alteration from Dimeric to Oligomeric State. Antioxidants 8 (2) 2019 (Impact factor-4.52; ISSN 2076-3921)
    
    
11. 11.Sharma GS, Warepam M, Bhattacharya R, Singh LR. Covalent Modification by Glyoxals Converts Cytochrome c Into its Apoptotically Competent State. Scientific Report 9 (1): 4781 2019 (Impact factor-4.52; ISSN 2076-3921)
    
    
12. 12.Dar PA, Mir SA, Bhat JA, Hamid A, Singh LR, Malik F, Dar TA. An anti-cancerous protein fraction from Withania somnifera induces ROS-dependent mitochondria-mediated apoptosis in human MDA-MB-231 breast cancer cells. Int J Biol Macromol. 135:77-87 2019 (Impact factor-4.8; ISSN: 0141-8130)
    
    
13. 13.Meesha Sharma, Shafikur Rahman, Sheeza Khan, Laishram Rajendrakumar Singh. The extracellular protein, transthyretin is an oxidative stress biomarker. Frontiers in Physiology 10 (5) 2019. (Impact factor-4.1; ISSN: 1664-042X)
    
    
14. 14.Tauheed Hasan, Reetika Arora, Aniket Kumar Bansal, Reshmee Bhattacharya and Laishram Rajendrakumar Singh. Disturbed homocystein metabolism is associated with cancer. Experimental and Molecular Medicine, 51(2):21, 2019. (Impact factor-5.06; ISSN 2092-6413)
    
    
15. 15.Hasan T, Kumari K, Devi SC, Handa J, Rehman T, Ansari NA, Singh L. R. Osmolytes in vaccine production, flocculation and storage: a critical review. Human Vaccine & Immunotherapeutics 15 (2) :514-525 (Impact factor-3.64; ISSN: 2164-554X)
    
    
16. 16.Sharma, Gurumayum Suraj, and Laishram Rajendrakumar Singh. "Conformational status of cytochrome c upon N-homocysteinylation: Implications to cytochrome c release." Archives of biochemistry and biophysics 614 (2017): 23-27. (Impact factor-3.60; ISSN 1522-4724)
    
    
17. 17.Sharma, G. S., and Singh L. R. "Polyols have unique ability to refold protein as compared to other osmolyte types." Biochemistry (Moscow) 82 (2017): 465-473. (Impact factor-1.9; ISSN: 1608-3040)
    
    
18. 18.Mohd Younus Bhat, Laishram Rajendrakumar Singh and Tanveer Ali Dar. Trimethylamine N-oxide abolishes the chaperone activity of ?-casein: an intrinsically disordered protein. (2017) Scientific Reports, 2017 26; 7 (1):6572. (Impact factor-4.52; ISSN 2076-3921)
    
    
19. 19.Rimpy Kaur Chowhan, Upendra Bhele, Hamidur Rahaman, and Laishram Rajendrakumar Singh. Understanding structural basis for redox regulation of Peroxiredoxin 6 using in silico approach. (2017) J. of Proteins and Proteomics. 8(4), pp. 195-204.