Manisha Tiwari


Bio-Organic Chemistry Laboratory
Email: mtiwari07@gmail.com manisha_07@hotmail.com

Dr Manisha Tiwari works focuses on the treatment of CNS and lifestyle disorders. The CNS disorders that we are working on include Alzheimer’s disease and epilepsy. Lifestyle disorders that we are researching include hyperlipidemia and cancer. Our laboratory works on the synthesis of small organic molecules for the treatment of these disorders. In the last 10 years we have synthesis several therapeutic agents for the treatment of Alzheimer’s Disease. One of the essential pharmacophores in our studies is aryl piperazine. We have prepared aryl piperazine derivatives, some of which have shown excellent activity against acetylcholinesterase and Abeta aggregation. In silico analysis of these derivatives including molecular docking studies and Molecular dynamics simulation studies have been carried out with A beta, which show strong interaction of these derivatives with the Abeta protein aggregates.

We have also prepared heterocyclic compounds for the treatment of cancer which have shown admirable activity in vivo

Honours and awards:

INSA visiting fellowship, 2004

Ongoing Projects:
  1. "The potential use of novel aryl and alkyl piperazine analogs for the treatment of Alzheimer’s disease: An in vivo and in vitro study”, funded by DST 2013-2016.

Selected Recent Publications:
  1. 34. Structure-based pharmacophore mapping and virtual screening of natural products to identify polypharmacological inhibitor against c-MET/EGFR/VEGFR-2. Journal of Biomolecular Structure and Dynamics, pp.1-15. Varma, D.A., Singh, M., Wakode, S., Dinesh, N.E., Vinaik, S., Asthana, S. and Tiwari, M., 2022

  2. 33. Crizotinib-induced anti-cancer activity in human cervical carcinoma cells via ROS-dependent mitochondrial depolarization and induction of apoptotic pathway. Journal of Obstetrics and Gynaecology Research.47(11) 3923-3930, 2021 Varma DA, Tiwari M.

  3. 32. Discovery of potent carbonic anhydrase inhibitors as effective anticonvulsant agents: drug design, synthesis, and in vitro and in vivo investigations. Mishra, C.B., Kumari, S., Angeli, A., Bua, S., Mongre, R.K., Tiwari, M. and Supuran, C.T., 2021.Journal of Medicinal Chemistry, 64(6), pp.3100-3114

  4. 31. The crystal structures of 2-(4-benzhydryl piperazin-1-yl)-N-(4-sulfamoylphenyl) Acetamide in complex with human carbonic anhydrase II and VII provide insights into selective CA inhibitor development. Katie D'Ambrosio,Anna DiFiore, Martina Buonanno, Shikha Kumari, Manisha Tiwari, Claudiu T Supuran, Chandra Bhushan Mishra, Simona Maria Monti & Giuseppira De Simone 2021 New Journal of Chemistry (Royal Society of Chemistry), 45(1), pp.147-152

  5. 30. Mishra, Chandra Bhushan?, Shruti Shalini?, Siddharth Gusain, Amresh Prakash, Jyoti Kumari, Shikha Kumari, Anita Kumari Yadav, Andrew M. Lynn, and Manisha Tiwari*. "Development of novel N-(6-methanesulfonyl-benzothiazol-2-yl)-3-(4-substituted-piperazin-1-yl)-propionamides with cholinesterase inhibition, anti-?-amyloid aggregation, neuroprotection and cognition enhancing properties for the therapy of Alzheimer's disease." RSC Advances 10, no. 30 (2020): 17602-17619.

  6. 29. Development of novel carbazole derivatives with effective multifunctional action against Alzheimer's diseases: Design, synthesis, in silico, in vitro and in vivo investigation., Chandra Bhushan Mishra, Siddharth Gusain, Shruti Shalini, Shikha Kumari, Amresh Prakash, Namrata Kumari, Anita Kumari Yadav, Jyoti Kumari, Kundan Kumar, Dr Manisha Tiwari*, Bioorg Chem. 2019 Dec 28; 95:103524

  7. 28. Naphthalene-triazolopyrimidine hybrid compounds: Synthesis, characterization, crystal structure, molecular docking and in vitro studies as potential multifunctional Anti-Alzheimer's agents, Tarana, Siddharth Gusain, Md Kausar Raza, Shruti Shalini, Jitendra Kumar, Manisha Tiwari, Nasimul Hoda, Bioorganic & Medicinal Chemistry;2019;27(14): 3156-3166

  8. 27. A multifunctional therapeutic approach: Synthesis, biological evaluation, crystal structure and Molecular docking of diversified 1H-pyrazolo[3,4-b]pyridine derivatives against Alzheimer's disease, Tarana Umar; Shruti Shalini; Md Kausar Raza; Siddharth Gusain; Jitendra Kumar Yadav; Manisha Tiwari and Nasimul Hoda., European Journal of Medicinal Chemistry:2019,175,2-19

  9. 26. New amyloid beta-disaggregating agents: Synthesis, pharmacological evaluation, crystal structure and molecular docking of N-(4-((7-chloroquinolin-4-yl)oxy)-3-ethoxybenzyl) amines. tarana umar, Shruti Shalini, Kausar Raza, Siddharth Gusain, Jitendra Kumar, Waqar Ahmed, Manisha Tiwari and Nasimul Hoda MedChemComm. 2018;9(11):1891-904

  10. 25. Discovery of potent anti-convulsant carbonic anhydrase inhibitors: Design, synthesis, in vitro and in vivo appraisal. Mishra CB, Kumari S, Angeli A Bua S, Buonanno M, Monti SM, Tiwari M*, Supuran CT* European Journal of Medicinal chemistry 2018 Jul 9;156:430-443. doi: 10.1016/j.ejmech.2018.07.019

  11. 24. Discovery of Benzenesulfonamide derivatives as carbonic anhydrase inhibitors with effective anticonvulsant action : Design, Synthesis, and Pharmacological evaluation Chandra Bhushan Mishra, Shikha Kumari, Andrea Angeli, Silvia Bua, Manisha Tiwari* and Claudiu T. Supuran* Journal of Medicinal Chemistry; (2018), 61: 3151-3165

  12. 23. The anti-epileptogenic and cognition enhancing effect of novel 1-[4-4- benzo {1,3} dioxol-5-ylmethyl-piperazin-1-yl)-phenyl]-3-phenyl-urea (BPPU) in pentylenetetrazole induced chronic rat model of epilepsy Chandra Bhushan Mishra, Shikha Kumari, Fouzia Siraj, Rajesh Yadav, Sweta Kumari, Ankit K. Tiwari, Manisha Tiwari* Biomedicine and Pharmacotherapy (2018), 105, 470-480

  13. 22. Discovery of novel Methylsulfonyl phenyl derivatives as potent human Cyclooxygenase-2 inhibitors with effective anticonvulsant action: Design, synthesis, in-silico, in vitro and in vivo evaluation Chandra bhushan mishra, Shikha Kumari, Amresh Prakash, R Yadav, AK Tiwari, P Pandey, Manisha Tiwari* European Journal of Medicinal Chemistry 2018, May10, 151;520-532

  14. 21. Novel Triazole-Piperazine Hybrid Molecules Induce Apoptosis via Activation of the Mitochondrial Pathway and Exhibit Anti-tumor Efficacy in Osteosarcoma Xenograft Nude Mice Model Chandra Bhushan Mishra Raj Kumar Mongre,; Shikha Kumari; Dong Kee Jeong ; Manisha Tiwari*: ACS Chemical Biology (accepted ) 2017 IF 5.09

  15. 20. Design, Synthesis, In Silico and Biological Evaluation of Novel 2-(4-(4-substituted piperazin-1-yl)benzylidene carboxamidesShikha Kumari, Chandra Bhushan Mishra, Danish Idrees, Amresh Prakash • Rajesh Yadav • Md. Imtaiyaz Hassan • Manisha Tiwari* Molecular Diversity 2016 (Accepted) IF 1.9

  16. 19. Novel Insights into Multitargeted Potential of N'-(4-benzylpiperidin-1-yl)alkylamine derivatives in the Management of Alzheimer's Disease Associated Pathogenesis Poonam Meena, Apra Manral, Manisha Tiwari* RSC Advances, 2016, 6, 104847 – 104867 Impact Factor 3.84

  17. 18. Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1 Rashi Arora, Sharad Sawney, Vikas Saini, Chris Steffi, Manisha Tiwari; Daman Saluja*, Molecular Cancer (2016) 15: 64 Springer Impact Factor 5.8

  18. 17. Design, Synthesis, in-Silico and Biological Evaluation of Novel Donepezil Derivatives as Multi-Target-Directed Ligands for the Treatment of Alzheimer's Disease Chandra Bhushan Mishra, Shikha Kumari Apra Manral and Manisha Tiwari* European Journal of Medicinal Chemistry 2016,135, 736-750 IF 3.9

  19. 16. Design, Synthesis and Evaluation of Novel Indandione Derivatives as Multifunctional Agents with Cholinesterase Inhibition, anti beta-amyloid Aggregation, Antioxidant Neuroprotection properties against Alzheimer's Disease: Chandra Bhushan Mishra, Apra Manral , Shikha Kumari, Vikas Saini, Manisha Tiwari Bioorganic & Medicinal Chemistry IF 2.9 (Elsevier) Bioorg Med Chem. 2016 Aug 15;24(16):3829-41.

  20. 15. Design, synthesis and biological evaluation of N-(5-methyl-isoxazol-3-yl/1,3,4-thiadiazol-2-yl)-4-(3-substitutedphenylureido) benzenesulfonamides as human carbonic anhydrase isoenzymes I, II, VII and XII inhibitors Chandra bhushan Mishra, Shikha Kumari, Andrea Angeli, SM Monti, M Buonanno, Amresh Prakash, Manisha Tiwari*, Claudiu T. Supuran* Journal of Enzyme Inhibition and Medicinal Chemistry, 2016 Accepted In Press (Taylor and Francis IF 3.43)

  21. 14. Synthesis and screening of triazolopyrimidine scaffold as multi- functional agents for Alzheimer's disease therapies Jitendra Kumar, Poonam Meena, Anju Singh, Ehtesham Jameel, Mudasir Maqbool, Mohammad Mobashir, Ashutosh Shandilya, ManishaTiwari*, Nasimul Hoda*, B. Jayaram European Journal of Medicinal Chemistry 2016 Aug 25;119:260-77

  22. 13. Development of cyanopyridine-triazine hybrids as lead multi-target anti-Alzheimer’s agents Mudasir Maqbool, Apra Manral, Ehteshaam Jameel, Jitendra Kumar, Vikas Saini, Ashutosh Shandilya, Manisha Tiwari*, Nasimul Hoda*, B.Jayaram IF 2.9 Elsevier Bioorg Med Chem. 2016 ;24(12):2777-88.

  23. 12. DADS analogs ameliorated the cognitive impairments of Alzheimer-like rat model induced by scopolamine Apra Manral, Poonam Meena, Vikas Saini, . Fouzia Siraj, Shruti Shalini, Manisha Tiwari,* Neurotoxicity Research (2016) 30:407–426 IF 3.538

  24. 11. Multifunctional Novel Diallyl Disulfide (DADS) derivatives with ß-Amyloid-reducing, Cholinergic, Antioxidant and Metal chelating properties for the Treatment of Alzheimer's Disease Apra Manral, Vikas Saini, Poonam Meena and Manisha Tiwari* Bioorganic and Medicinal Chemistry Impact factor 2.95 Volume 23, Issue 19, 2015, Pages 6389-6403

  25. 10. Hyperhomocysteinemia: Impact on Neurodegenerative diseases Meenakshi Sharma, Manisha Tiwari and Rakesh Kumar Tiwari Basic and Clinical Pharmacology and Toxicology 117(5), 287-96, 2015 IF 2.124 (wiley)

  26. 9. Design, Synthesis and Pharmacological evaluation of N-[4-(4-(alkyl/aryl/heteroaryl/)-piperazin-1-yl)-phenyl]-carbamic acid ethyl ester derivatives as novel anticonvulsant agents Shikha Kumari, Chandrabhushan Mishra and Manisha Tiwari* Bio-organic and Medicinal Chemistry Letters 25 , 1092-1099, 2015 Impact Factor 2.33 (Elsevier)

  27. 8. Synthesis, biological evaluation and molecular docking study of novel piperidine and piperazine derivatives as multi-targeted agents to treat Alzheimer's disease Poonam Meena, Apra Manral, Manisha Khatri, Vishal Nemaysh, Pratibha M. Luthra and Manisha Tiwari* Bioorganic and Medicinal Chemistry 23, 1135-1148, 2015 Impact factor :2.95(Elsevier)

  28. 7. Thiazole: A promising heterocycle for the development of potent CNS active agents (Review Article) Chandra Bhushan Mishra, Shikha Kumari and Manisha Tiwari* European Journal of Medicinal Chemistry 92, 1-34, 2015 Impact factor 3.43 (Elsevier)

  29. 6. Protective effects of a Piperazine derivative [N-{4-[4-(2-methoxy phenyl)-piperazin-1-yl]-phenyl} Carbamic acid ethyl ester] against aluminium induced neurotoxicity: Insights from in silico and in vivo studies Poonam Meena, Apra Manral, Vikas Saini and Manisha Tiwari* Neurotoxicity Research 27, 314-327, 2015 Impact Factor 3.54 (Springer)

  30. 5. Meena,P., Manral, A., Saini, V., and Tiwari M (2014).Protective effects of a Piperazine derivative [N-{4-[4-(2-methoxy phenyl)-piperazin-1-yl]-phenyl} Carbamic acid ethyl ester] against aluminium induced neurotoxicity: Insights from in silico and in vivo studies. Neurotoxicity Research (online November 18th, 2014).

  31. 4. Alam, S., Khatri, M., and Tiwari , M. (2013). Assessment of the antioxidant activity of aqueous extract of Acacia catechu bark:An in vitro and in vivo study.Journal of Pharamacy Research, 1(1),109-114.

  32. 3. Novel diallyldisulfide analogs ameliorate cardiovascular remodeling in rats with L-NAME –induced hypertension. Dinesh Sharma, Apra Manral, Vikas Saini, Avninder Singh, BP Srinivasan and Manisha TiwariEuropean Journal of Pharmacology ; 691, 198-208 ,2012

  33. 2. In vitro cytotoxic activity of methanolic extract of the bark of Acacia catechu against human oral and lung cancer cell lines Sameena Alam, Manisha Khatri and Manisha Tiwari Journal of Pharmacy Research ,5(8), 4487-4491, 2012

  34. 1. Synthesis and Pharmacological Evaluation of [(4-Arylpiperazin-1-yl)-alkyl]-carbamic Acid Ethyl Ester Derivatives as Potential Anxiolytic Agents Manisha Khatri, Santosh Kumar Rai, Ranjit Ranbhor, Krishna Kishore, Manisha Tiwari Archives of Pharmacal Research, 35(7), 1143-1152, 2012