Faculty


Manisha Tiwari

Professor

Bio-Organic Chemistry Laboratory
Email: mtiwari07@gmail.com manisha_07@hotmail.com

Our lab has focused on development of small heterocyclic molecules for the treatment of neurodegenerative disorders, namely Alzheimer's Disease and Epilepsy. At present our lab is conducting research on treatment of Amyotrophic Lateral Sclerosis ( a motor neuron disease)

In pursuance of this aim, we have synthesized new heterocyclic molecules. These are known to be neuroprotective. In ALS, the hSOD1 gene gets mutated. As a result the protein tends to get aggregated. The most common mutations include G85R and A4V. We have used plasmids which contain human SOD 1 gene and carried out site directed mutagenesis. These were used to express mutant protein. The inhibitory activity of the test compounds (4-19) against SOD1 mutant protein has been assessed by employing Thioflavin T (ThT) based fluorescence assays. The activity of the compounds has been further assessed, by employing Electron microscopy. The therapeutic effect of these molecules , is also tested in a neuronal cell line based model. The cytotoxicity of the two most active compounds was studied on Neuro 2A cells, using MTT assay. the potential of SG-9 and SG-10 to rescue Neuro-2a cells against hSOD1 G85R associated cytotoxicity was evaluated. The most active compounds have shown 70 to 90% inhibition of protein aggregation A patent has been filed for this work.

Honours and awards:

INSA visiting fellowship, 2004

Patents Granted:
  1. 01. Siddharth Gusain, Shruti Shalini, Diksha Varma, Manisha Tiwari, Chandra Bhushan Mishra, Shikha Kumari "A method for designing and synthesizing carbazole based novel multifunctional agents as potential Anti-Alzheimer's' agents South African Patent granted 23/2/2022 Patent Number 2021/09489

  2. 02. Siddharth Gusain, Shikha kumara, Chandra Bhushan Mishra, Shruti Shalini, Manisha Tiwari, Diksha varma "A system for developing a series of 1-Phenyl-3/4-[4-(Aryl/Heteroaryl/ Alkyl-piperazine-1-yl) Phenyl Urea derivatives as Anticonvulsant and Antidepressant" agents Patent number IPC C07D 241/04 German Patent Granted 23/5/2022

  3. 03. Manisha Tiwari, Siddharth Gusain, Dinesh Sharma, Patent Number 2023/02262, Republic of South Africa, Patent Granted 31/5/2023

  4. 04. Manisha Tiwari, Dr Siddharth Gusain, CB Mishra, Kajal yadav, Title: METHOD FOR SYNTHESIZING AND CHARACTERIZING CARBAZOLE DERIVATES AS MUTANT HSOD1 AGGREGATION INHIBITORS, Application Number 2024/05217.

Selected Recent Publications:
  1. 01. Multitarget action of benzothiophene-piperazine small hybrid molecule against Alzheimer's Disease:In silico, In vitro and in vivo investigation CB Mishra, Shruti Shalini, Siddharth Gusain, Pawan Kumar.Raok Jeon and Manisha Tiwari Biomedicine and Pharmacotherapy, pp 116484, 174, 2024 Impact factor 7.5 (May 2024)

  2. 02. Synthesis and characterization of Fe3O4@SiO2@PDA@Ag core-shell nanoparticles And biological application on human lung cancer cell line and antibacterial strains Snigdha Singh, Tanya Goel , Aarushi Singh, Heerak Chugh, Indrajit Roy, Manisha Tiwari and Ramesh Chandra Published online DEC 2023 Artificial cells, Nanomedicine and Biotechnology Impact factor 4.5

  3. 03. Structure-based pharmacophore mapping and virtual screening of natural products to identify polypharmacological inhibitor against c-MET/EGFR/VEGFR-2. Journal of Biomolecular Structure and Dynamics, pp.1-15. Varma, D.A., Singh, M., Wakode, S., Dinesh, N.E., Vinaik, S., Asthana, S. and Tiwari, M., 2022. (Impact factor 2.7)

  4. 04. Discovery of potent carbonic anhydrase inhibitors as effective anticonvulsant agents: drug design, synthesis, and in vitro and in vivo investigations. Mishra, C.B., Kumari, S., Angeli, A., Bua, S., Mongre, R.K., Tiwari, M. and Supuran, C.T., 2021. Journal of Medicinal Chemistry, 64(6), pp.3100-3114. (Impact Factor 7.3)

  5. 05. The crystal structures of 2-(4-benzhydryl piperazin-1-yl)-N-(4-sulfamoylphenyl) Acetamide in complex with human carbonic anhydrase II and VII provide insights into selective CA inhibitor development. Katie D'Ambrosio,Anna DiFiore, Martina Buonanno, Shikha Kumari, Manisha Tiwari, Claudiu T Supuran, Chandra Bhushan Mishra, Simona Maria Monti & Giuseppira De Simone New Journal of Chemistry (Royal Society of Chemistry), 45(1), pp.147-152. (Impact Factor 3.3)

  6. 06. Development of novel N-6-methanesulfonyl-benzothiazol-2-yl)-3-(4-substituted piperazin-1-yl) propionamides with cholinesterase inhibition, antibetaamyloid aggregation, neuroprotection and cognition enhancing properties for the therapy of Alzheimer's Disease RSC Advances. 17602-17619, 10 (30), 2020 (Impact factor 3.1)

  7. 07. Progress in the development of human carbonic anhydrase inhibitors and their pharmacologic applications. Where are we Today? Chandrabhushan Mishra, Manisha Tiwari and Claudiu T. Supuran Medicinal research reviews 2485-2565, 40(6), 2020 (Impact factor 9.3)

  8. 08. Microbial biofilm ecology, in silico study of quorum sensing receptor-ligand interactions and biofilm mediated bioremediation. Balan, B., Dhaulaniya, A.S., Varma, D.A., Sodhi, K.K., Kumar, M., Tiwari, M. and Singh, D.K., 2020. Archives of Microbiology, pp.1-18. (Impact Factor 1.607)